Detection of Β-Lactamase OXA Genes and Biofilm Production in Carbapenem Resistant Gram-Negative Bacilli

Objectives: The emergence of carbapenem resistance in biofilm producer Gram-negative bacteria poses a substantial challenge to manage infections. This study aimed evaluate production and molecular characterization rods. Methods: Total 46 were isolated from various clinical samples, including blood, CSF, urine, sputum, saliva, tracheal secretion, pus. Following the standard guidelines for bacterial identification (CLSI), pathogens are identified. antibiotic susceptibility profile was obtained against commercially available antibiotics Amoxicillin, Amikacin, Ceftazidime, Cefepime, Imipenem, Meropenem, Gentamycin, Tobramycin, Doxycycline, Minocycline, Tazobactam, Ciprofloxacin, Levofloxacin following CLSI guidelines. Biofilm potential quantified by using 96-well flat bottom microtiter plates. Phenotypic carbapenem-resistant performed. genotypic determined OXA-23, OXA-24, OXA-51 OXA-58 gene primers. Results: strains found as Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae, Serratia marcescens. phenotypic drugs 95%. DNA kit polymerase chain reaction assay performed optimized identify OXA-23 (84%), OXA-24(90%), OXA-51(83%) OXA-58(80%) strains. Practical Implication: For better prompt management antimicrobial further work on genetics is required warranted produce effective enhance effect carbapenems. Conclusion: prevalence among bacilli high. OXA genes have raised concern detect carbapenemase-producing bacteria. Keywords: Antibiotic Resistance, Biofilm, Carbapenem-resistance, marcescens, β-lactamases,